Project number
16102
Organization
UA Department of Chemical and Environmental Engineering
Academic year
2016-2017
The goal of this project is to design a process for the continuous production of Enbrel, an arthritic drug that is an active biologic. The drug is a fusion protein secreted by live Chinese hamster ovary cells and is batch-made in a bioreactor.
Modeling the system with several reactors allowed the team to transform the process from batch to continuous. From the bioreactors, cells are centrifuged before being filtered to remove cellular components.
The protein of interest, etanercept, is further separated by affinity chromatography before viral inactivation and more chromatography steps. The protein goes through viral filtration and diafiltration before it becomes the final product.
Modeling the system with several reactors allowed the team to transform the process from batch to continuous. From the bioreactors, cells are centrifuged before being filtered to remove cellular components.
The protein of interest, etanercept, is further separated by affinity chromatography before viral inactivation and more chromatography steps. The protein goes through viral filtration and diafiltration before it becomes the final product.